Figure 4. Acute L-arginine supplementation activates mTORC1-S6K1 signaling, but not Arg-II expression/activity.

Endothelial cells were treated as described in Fig. 2. (A) Western Blots showing activation of mTORC1 and S6K1 as monitored by phosphorylation of S6K1-T389 and S6-S235/236, respectively. (B) Western Blot analysis of Arg-II expression and arginase activity assay. The graphics at the corresponding right panels present the quantification of the signals. *p < 0.05 vs 0.1 mmol/L