Figure 6. Arg-II and S6K1 mediate the chronic effect of L-arginine on cell senescence.

Endothelial cells were treated as in Fig. 5. (A) SA-β gal assay. (B) Western blot analysis of eNOS, ICAM-1, VCAM-1. Tubulin served as loading control. (C) DHE staining for detection of O₂^.−, DAF-2DA staining for detection of NO. The graphics at the corresponding right panels present the quantification of the signals. *p < 0.05 vs rAd/U6-LacZshRNA control+0.5 mmol/L L-arginine. Scale bar = 200 μm