Figure 1.

Expression of TLRs in human prostate cancer cell lines. A: RT-PCR showing expression of TLR1-10, MyD88 and CD14 transcripts. RT-PCR was performed according to the protocol described in Materials and methods. PBMC served as positive control. Amplification of Beta-actin in the same samples was used as loading control. No amplification controls (NC)(no reverse transcriptase added) were included as negative controls in all PCR programs. B: Western blot analysis of TLR2 and TLR4 expression. Positive sample and loading controls were included as RT-PCR experiments. Lanes in which primary antibodies were substituted by non-immune sera served as negative controls (NC). Similar results were observed in three independent experiments.