Table 1.
Sequence, amplicon size and annealing temperatures of primers used in this study
| Forward (5′–3′) | Reverse (5′–3′) | Product size (bp) | Annealing Temp(°C) | |
|---|---|---|---|---|
|
TLR1 |
CTATACACCAAGTTGTCAGC |
GTCTCCAACTCAGTAAGGTG |
220 |
60 |
|
TLR2 |
GTACCTGTGGGGCTCATTGT |
CTGCCCTTGCAGATACCATT |
191 |
62 |
|
TLR3 |
GATCTGTCTCATAATGGCTTG |
GACAGATTCCGAATGCTTGTG |
305 |
60 |
|
TLR4 |
GAGCTTTAATCCCCTGAGGCA |
GATTGGATAAGATTGTGAGCC |
306 |
54.8 |
|
TLR5 |
CTAGCTCCTAATCCTGATG |
CCATGTGAAGTCTTTGCTGC |
438 |
59 |
|
TLR6 |
AGGTGCCTCCATTATCCTCA |
GAATCCATTTGGGAAAGCAG |
211 |
59 |
|
TLR7 |
CATGCTCTGCTCTCTTCAACC |
CGATCACATGGTTCTTTGGA |
201 |
59 |
|
TLR8 |
GCCAGCGAGTCTCACTGAACT |
GCCAGGGCAGCCAACATA |
558 |
61 |
|
TLR9 |
TTCCCTGTAGCTGCTGTCC |
ACAGCCAGTTGCAGTTCACC |
207 |
58 |
|
TLR10 |
GGCCAGAAACTGTGGTCAAT |
CTGCATCCAGGGAGATCAGT |
199 |
61 |
|
CD14 |
AGAGTTCACAAGTGTGAAGC |
CCTTGACCGTGTCAGCATA |
373 |
58 |
|
MyD88 |
GACCCAGCATTGAGGAGGATTG |
GCTTCTGATGGGCACCTGGAGAG |
435 |
60 |
| βactine | GTGGGGCGCCCCAGGCACCA | CTCCTTAATGTCACGCACGATTTC | 538 | 60 |