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Expression and purification of rCsproSERPIN3 by 12% SDS–PAGE stained with Coomassie brilliant blue. Protein molecular weight markers (M), lysate of E. coli with pET28a(+) before induction (lane1) and after induction (lane 2), lysate of E. coli with pET28a(+)-CsSERPIN3 before induction (lane 3) and after induction (lane 4), supernatant (lane 5), and precipitant (lane 6) of lysate of E. coli with pET28a(+)-CsSERPIN3 after induction; lane 7, rCsproSERPIN3 after purification.
