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. 2014 Jun;108(4):179–185. doi: 10.1179/2047773214Y.0000000138

Figure 3.

Figure 3

Western blot analysis of rCsproSERPIN3. Protein molecular weight markers; rCsproSERPIN3 probed with naive rat serum (lane 1), with anti-rCsproSERPIN3 rat serum (lane 2), with serum from rat infected with C. sinensis (lane 3), with serum from normal human (lane 4), with serum from human infected with C. sinensis (lane 5); lysate of E. coli with pET28a(+)-CsSERPIN3 before induction (lane 6) and after induction (lane 7) probed with anti-rCsproSERPIN3 rat serum; lysate of E. coli with pET28a(+)-CsSERPIN3 before induction (lane 8) and after induction (lane 9) probed with His monoclonal antibody of mouse; rCsproSERPIN3 after purification probed with His monoclonal antibody of mouse (lane 10).