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. 2014 Jun;108(4):179–185. doi: 10.1179/2047773214Y.0000000138

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© W. S. Maney & Son Ltd 2014

PMC Copyright notice

Transcript analysis of CsSERPIN and CsSERPIN3 at the stage of adult and metacercaria of Clonorchis sinensis. Beta-actin of C. sinensis (accession number: EU109284) was used as the transcription control. The ΔC_t values of each adult and metacercaria were calculated by deducting the values of beta-actin, and the –ΔΔC_t values were figured out according to the method. The amplification of CsSERPIN3 of adult worms was employed as the calibrator to evaluate relative expression levels of the others. Each bar represented the mean value from three experiments with standard deviation (*P<0.05).

Inline graphic — Transcript analysis of CsSERPIN and CsSERPIN3 at the stage of adult and metacercaria of Clonorchis sinensis. Beta-actin of C. sinensis (accession number: EU109284) was used as the transcription control. The ΔC_t values of each adult and metacercaria were calculated by deducting the values of beta-actin, and the –ΔΔC_t values were figured out according to the method. The amplification of CsSERPIN3 of adult worms was employed as the calibrator to evaluate relative expression levels of the others. Each bar represented the mean value from three experiments with standard deviation (*P<0.05).