Table 1.
Optimization of experimental factors affecting the chromatographic performance of the proposed method
|
Parameter |
Number of theoretical plates (N) |
Tailing factor |
Resolution (Rs) |
||
|---|---|---|---|---|---|
| TS | JM | TS | JM | TS/JM | |
| Organic modifier nature | |||||
| Methanol |
7566 |
12598 |
1.06 |
1.10 |
11.10 |
| 1-Propanol |
5974 |
12154 |
1.16 |
0.20 |
16.40 |
| Acetonitrile |
4253 |
9098 |
1.10 |
0.10 |
14.10 |
| Methanol concentration (%) | |||||
| 8 |
3791 |
9496 |
1.05 |
1.09 |
9.97 |
| 10 |
5566 |
11598 |
1.06 |
1.10 |
11.10 |
| 12 |
4770 |
10204 |
1.17 |
1.24 |
11.42 |
| 14 |
7312 |
12723 |
1.09 |
1.10 |
11.16 |
| 16 |
4549 |
9675 |
1.21 |
1.31 |
11.80 |
| SDS concentration (M) | |||||
| 0.10 |
4317 |
8931 |
1.20 |
1.24 |
12.72 |
| 0.12 |
5800 |
9848 |
1.20 |
1.28 |
12.07 |
| 0.15 |
5312 |
10723 |
1.21 |
1.21 |
11.67 |
| 0.17 |
7516 |
12973 |
1.09 |
1.13 |
10.81 |
| 0.20 |
3429 |
10814 |
1.19 |
1.05 |
10.76 |
| pH | |||||
| 2.5 |
4247 |
9564 |
1.18 |
1.24 |
12.83 |
| 3.0 |
5985 |
10611 |
1.17 |
1.23 |
12.89 |
| 4.0 |
7516 |
12973 |
1.08 |
1.13 |
10.84 |
| 5.0 | 4830 | 11349 | 1.19 | 1.14 | 11.80 |