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. 2014 Jun 25;34(26):8904–8917. doi: 10.1523/JNEUROSCI.1146-14.2014

Figure 1.

Figure 1.

Age-related impairment in the induction of IL-4Rα surface expression on microglia after SCI. Adult female (3–4 months) and aged female (18–22 months) BALB/c mice were subjected to a mid-thoracic (T9) laminectomy (Lam) or SCI. After 1 dpi or 3 dpi, enriched CD11b+ cells from the spinal cord were isolated. Representative bivariate dot plots of CD11b/IL-4Rα labeling in microglia from (A) adult and (B) aged mice. Gating was based on age-matched isotype controls. C, Average percentage of IL-4Rα+ microglia (n = 4–5). D, IL-4 and (E) IL-13 mRNA expression was determined from two 1 mm sections of spinal cord collected adjacent to the lesion epicenter (n = 4–5). Error bars represent the mean ± SEM. Means with *p < 0.05 are significantly different and means with +p = 0.1 tend to be different from Adult-Lam controls. Means with #p < 0.05 are significantly different from Adult-SCI-3 dpi.