Figure 7.

IL-4-mediated reprogramming of activated microglia supported neurite outgrowth ex vivo. Adult (3 months) male BALB/c mice received an intraperitoneal injection of saline or LPS and 4 h later enriched microglia were isolated. Enriched microglia were plated in transwell inserts directly over DRG neurons collected from adult (3 months) male BALB/c mice. Cultures were treated with vehicle or IL-4 for 24 h. A, Representative images of β-tubulin-III staining of DRG neurons. B, β-tubulin-III proportional area as a percentage of Saline-Vehicle (n = 6–8). Error bars represent the mean ± SEM. Means with *p < 0.03 are significantly different and means with ⁺p = 0.1 tend to be different from Saline-Vehicle controls.