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. 2014 Jun 25;34(3):e00116. doi: 10.1042/BSR20140062

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) WT and (C) CL-Pgp ATPase activity was assayed with purified proteins in the presence of increasing concentrations of verapamil. The solid lines represent non-linear regression analyses to the data points (see the Materials and Methods section) No cooperativity was observed with Hill coefficients close to 1.0 (1.04 and 1.00, respectively). Each data point represents the mean from at least three independent experiments±S.D.. (B) WT and (D) CL- Pgp purified proteins were assayed in the presence of 30 μM verapamil to maximally stimulate ATPase activity, but with increasing concentrations of the inhibitor cyclosporine A. The solid lines are non-linear regression fits, Hill coefficients were 1.40 and 1.22, respectively.