Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Bone. 2014 May 2;64:273–280. doi: 10.1016/j.bone.2014.04.028

Figure 2.

Figure 2

Effect of Notch on Ctgf transcript stability in osteoblasts. Calvarial osteoblasts isolated from RosaNotch mice were transduced with Ad-CMV-Cre, to activate Notch (filled circles), or control Ad-CMV-GFP (open circles) and cultured. Seventy-two h after confluence, cells were transcriptionally arrested by the addition of DRB (time 0), and harvested at the indicated times after DRB. Total RNA was extracted, reversed transcribed and amplified by qRT-PCR. Values are means ± SEM; n = 11 to 12. Data are expressed as percent of Ctgf mRNA corrected for Rpl38 expression, relative to the time of DRB addition and plotted versus time, and were pooled from 3 independent experiments. Slopes from Notch activated and control cells are significantly different, p < 0.05.