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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Bone. 2014 May 2;64:273–280. doi: 10.1016/j.bone.2014.04.028

Figure 5.

Figure 5

Effect of Notch on Hey2 mRNA, Ctgf mRNA and Ctgf hnRNA expression in osteoblasts. Wild type calvarial osteoblasts were cultured on plates coated with the Notch ligand Delta like 1 (Dll1) at the indicated doses for 72 h following confluence. Total RNA was extracted, reverse transcribed and amplified by qRT-PCR. Data are expressed as copy number of Hey2 mRNA, Ctgf mRNA and Ctgf hnRNA corrected for Rpl38 expression. Values are means ± SEM; n = 4. *Significantly different from control, p < 0.05.