Table 1.
Primers used for qRT-PCR assay determinations. GenBank accession number identifies transcript recognized by primer pairs.
| qRT-PCR | |||
|---|---|---|---|
| Gene | Strand | Sequence 5'–3' | GenBank Accession Number |
| Ctgf mRNA | Forward | CTGCCTGGGAAATGCTGCGAGGAGT | NM_010217 |
| Reverse | GTTGGGTCTTGGGCCAAATGT | ||
| Ctgf hnRNA | Forward | ACCCTACGCCTGACCTACAA | NC_000076 |
| Reverse | CATCTTTGGCTGCGGGAGAG | ||
| Gapdh | Forward | CCCCTCTGGAAAGCTGTGGCGT | NM_008084 |
| Reverse | AGCTTCCCGTTCAGCTCTGG | ||
| Hes1 | Forward | ACCAAAGACGGCCTCTGAGCACAGAAAGT | NM_008235 |
| Reverse | ATTCTTGCCCTTCGCCTCTT | ||
| Hey1 | Forward | ATCTCAACAACTACGCATCCCAGC | NM_010423 |
| Reverse | GTGTGGGTGATGTCCGAAGG | ||
| Hey2 | Forward | AGCGAGAACAATTACCCTGGGCAC | NM_013904 |
| Reverse | GGTAGTTGTCGGTGAATTGGACCT | ||
| Rbpjκ | Forward | ACAGACAAGGCAGAATACAC | NM_001080928 |
| NM_009035 | |||
| Reverse | CAACTGAAGACTTTCTACGA | NM_001080927 | |
| Rpl38 | Forward | AGAACAAGGATAATGTGAAGTTCAAGGTTC | NM_001048057 |
| NM_023372 | |||
| Reverse | CTGCTTCAGCTTCTCTGCCTTT | NM_001048058 | |