Table 2.
Effect of Notch on the activity of Ctgf promoter and pMIR-Ctgf 3'UTR constructs.
| 12xCSL-Luc | 3.8 kb Ctgf-Luc | |
|---|---|---|
| Luciferase/β-galactosidase | ||
| A. | ||
| Control | 0.4 ± 0.4 | 1854 ± 250 |
| Notch | 19.7 ± 7.5* | 1489 ± 393 |
| B. | ||
| Control | 0.4 ± 0.1 | 190 ± 9 |
| pcDNA-NICD | 127 ± 15 | 141 ± 18* |
|
pMIR-Ctgf 3'UTR Luciferase/β-galactosidase |
||
| C. | ||
| Control | 1637 ± 219 | |
| Notch | 232 ± 22* | |
Calvarial osteoblasts isolated from RosaNotch mice were transduced with Ad-CMV-Cre (Notch) or Ad-CMV-GFP (Control) and transfected in A. with a 12xCSL-Luc reporter or a 3.8 kb Ctgf-Luc promoter construct and in C. with pMIR-Ctgf 3'UTR reporter. In B. wild type osteoblasts were co-transfected with pcDNA-NICD expression construct or control pcDNA3.1 and 12xCSL-Luc or Ctgf-Luc promoter fragment. Values are means ± SEM; n = 6 of luciferase activity corrected for β-galactosidase activity.
*
Significantly different from control, p < 0.05