Figure 5.

Verification of identified protein binding with Nogo-C. Co-immunoprecipitation and intracellular co-localization between identified proteins and Nogo-C were performed. A: Myc-Nogo-C and HA-Nogo-B. B: HA-Nogo-C and Myc-B-Raf. Both forward and reciprocal immunoprecipitations were performed using anti-HA or anti-Myc monoclonal antibody. C: Co-localization of exogenous Myc-Nogo-B and Nogo-C-GFP. D: Co-localization of exogenous Myc-B-Raf and Nogo-C-GFP. primary anti-Myc monoclonal antibody and Alexa Fluor 488-congulated secondary antibody were used. SMMC-7721 cells were observed by confocal laser scanning microscope. Bars represent 10 μm.