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Effect of Rhy on the expression of P-gp and MDR1. Caco-2 cells were treated with indicated concentration of RhyA or verapamil (Ver, 50 μmol/L) for 24 h. The cells were collected to detect protein expression of P-gp and MDR1 with Western blot (A) and flow cytometry (B), respectively (n = 3).
