Figure 4.
Reduction of miR-9 and miR-181a can enhance RAW264.7 cells migration and raise mouse primary OCs survival rate. A: RAW264.7 cells were transfected with pre-miR control, miR-9/181a mimics, anti-miR control or miR-9/181a inhibitor, respectively. Cells were harvested 48 h after transfection and recounted to 0.5×105 cells/ml in every group to seed Transwells for cell migration assay. At time of harvest, the cells on top of the membranes were removed, and the cells on the bottoms of the membranes were stained with hematoxylin and eosin. The cell migration was quantified by counting the amount of cells passing through the membrane from five different fields per sample at 200× selected in a random manner after 12 h of incubation. A show the representative photomicrographs of cells passing through the membrane at ×200 original magnification. Data are expressed as the mean numbers of independent triplicate experiments. *P<0.05. B: OCs were purified 6 h after the infection and some of the cultures were subjected to tartrate-resistant acid phosphatase (TRAP) staining. Cell viability/survival is expressed as morphologically intact TRAP-positive multinucleated cells. Other cultures were further incubated for the indicated times, and then the number of living OCs was counted. The number of viable cells remaining at the different time points is shown as a percentage of the cells at time zero.