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RhTSLP stimulates the secretion of MCP-1 and IL-8 of ESCs. ESCs (n=6) (1×105 cells/well) were seeded in 24-well plates, and treated with rhTSLP (0-100 ng/ml) for 48 h, and then ELISA was performed to analyze the secretion level of MCP-1 (A), IL-8 (B) and IL-6 (C) in the supernatant. Data are expressed as the mean ± SD. P<0.05, **P<0.01 and ***P<0.001 as compared to control.
