Table 4.
Chemically Defined Media for Growth of S. aureus Cultures.
| Solution(s) | Item | Amount (g) | Volume (ml) | Solvent | Sterilize |
|---|---|---|---|---|---|
| Salt Solution | K2HPO4 | 70 | 1000 | H2O | Autoclave |
| KH2PO4 | 20 | ||||
| (NH4)2SO4 | 10 | ||||
| MgSO4⬆7H2O | 25.6 | ||||
| Amino Acids | Phe | 1 | 250 | 1M NH4OH | Autoclave |
| Iso | 7.50E-01 | 250 | 1M NH4OH | Autoclave | |
| Tyr | 1.25 | 250 | 1 N HCl | Autoclave | |
| Cys★ | 5.00E-01 | 250 | 1 N HCl | Autoclave | |
| Glu | 2.5 | 250 | 1 N HCl | Autoclave | |
| Lys | 2.50E-01 | 250 | 1 N HCl | Autoclave | |
| Met | 1.75 | 250 | 1 N HCl | Autoclave | |
| His | 7.50E-01 | 250 | 1 N HCl | Autoclave | |
| Trp | 2.50E-01 | 250 | 1 N HCl | Autoclave | |
| Leu | 2.25 | 250 | 1 N HCl | Autoclave | |
| Asp | 2.25 | 250 | 1 N HCl | Autoclave | |
| Arg | 1.75 | 250 | 1 N HCl | Autoclave | |
| Ser | 7.50E-01 | 250 | dH2O | Autoclave | |
| Ala | 1.5 | 250 | dH2O | Autoclave | |
| Thr | 7.50E-01 | 250 | dH2O | Autoclave | |
| Gly | 1.25 | 250 | dH2O | Autoclave | |
| Val | 2 | 250 | dH2O | Autoclave | |
| Pro | 2.50E-01 | 250 | dH2O | Autoclave | |
| Bases★★ | Adenine | 1.25E-01 | 250 | dH2O | Autoclave |
| Cytosine | 1.25E-01 | 250 | dH2O | Autoclave | |
| Guanine | 1.25E-01 | 250 | dH2O | Autoclave | |
| Thymine | 5.00E-01 | 250 | dH2O | Autoclave | |
| Uracil | 1.25E-01 | 250 | dH2O | Autoclave | |
| Vitamin Solution | Thiamine | 1.00E-01 | 100 | H2O | Autoclave |
| Niacin | 1.20E-01 | ||||
| Biotin | 5.00E-04 | ||||
| Ca Pantothenate | 2.50E-02 | ||||
| Trace Elements | FeCl3 | 8.00E-01 | 100 | H2O | Filter Sterilize |
| ZnCl | 6.95E-03 | ||||
| MnCl ⬆ 4H20 | 9.90E-03 | ||||
| Boric Acid | 6.00E-04 | ||||
| CoCl2 ⬆ 6H20 | 3.97E-02 | ||||
| CuCl2 ⬆ 2H20 | 2.56E-04 | ||||
| NiCl2 ⬆ 6H20 | 2.38E-03 | ||||
| Na2MoO4 ⬆ 2H20 | 3.58E-03 |
★
Cystine not cysteine
★★
May need to heat or titrate using KOH to get into solution