Table 1.

Simulated intra-Golgi transport of albumin by diffusion, via intercisternal continuities, occurs in the timescale of seconds

DOI: http://dx.doi.org/10.7554/eLife.02009.019

Length of tubule (nm)	Diameter of tubule (nm)	Number of cisterna	Time needed for 90% equilibration (s)
100	30	6	14.9
100	60	6	7.8
100	120	6	6.8
50	30	6	11.4
150	30	6	19.1
100	30	4	6.4
100	60	4	3.4
100	120	4	3.0
50	30	4	5.0
150	30	4	8.4

The size and geometry of the Golgi stack used for the simulations is defined in the legend to Figure 6. The variable parameters used for the simulation are: length of tubules (from 50 to 150 nm), diameter of tubules (from 30 to 120 nm), and number of cisternae (between 4 and 6). The tubules here refer to the intercisternal tubules connecting two cisternae of a Golgi stack. The time needed for 90% equilibration of albumin across the Golgi stack under varying combinations of the indicated parameters was computed. As can be seen from the data, equilibration across the cisternae happens in seconds across all the conditions.