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Confocal fluorescence microscopy images of GUVs after 2 h incubation in buffer containing BODIPY 492/515. (A) SM/PE/Ch (2:1:1) + buffer. (B) SM/PE/Ch (2:1:1) + 1.4 μM sphingosine. (C) Assay of sphingosine-induced influx of BODIPY into GUV (t = 2 h). Black bars: SM/PE/Ch (2:1:1), gray bars: SM/PE/Ch (2:1:1) + 5 mol % PA. Average values ±S.D. of 90 vesicles. To see this figure in color, go online.
