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The distribution of GUVs as a function of the degree of filling after 2 h incubation with sphingosine is shown in GUVs made of (A) SM/PE/Ch (2:1:1) and (B) SM/PE/Ch (2:1:1) + 5% PA. Sphingosine concentration: (
) 0 μM, (X) 0.2 μM, (♦) 0.6 μM, (✚) 1 μM, (×) 1.3 μM. Average values ± SD of 90 vesicles.
