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. 2014 Jun 16;14:167. doi: 10.1186/1471-2229-14-167

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Copyright © 2014 Liu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Nodulation and growth phenotypes of M. truncatula plants after inoculation with S. meliloti strain Rm41. A, F83005.5 and A20 lines after inoculation by S. meliloti strain Rm41. A20 (right) grew normally under nitrogen-free conditions, whereas F83005.5 (left) can hardly survive under the same conditions. B, Nodulation phenotypes of the same plants in panel A. A20 roots formed nitrogen-fixing root nodules (right), while F83005.5 cannot nodulate with Rm41. C and D, a closer look at the nodulation phenotypes of A20 and F83005.5, showing fully developed functional nodules on the roots of A20 (D) and the nodule primordial (arrows) formed on the roots of F83005.5 (C).