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. 2014 Jun 17;15:10. doi: 10.1186/1471-2091-15-10

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Copyright © 2014 Yonemoto et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Purified protein activity assay. Left: Hydrogen evolution activities of G1, C258D, and C295D after purification using IMAC/strep-tactin tandem purification. Error bars represent the standard error of the mean activity. Right: anti-HynL and anti-strep western blots of enzyme samples.