Figure 1.
IH in whole-cell voltage-clamped ventricular myocytes at pH 6.0. (A) Currents induced by pulses from −40 mV to the indicated voltages in a ventricular myocyte bathed by 150 mM NMG external solution at pH 6.0 (upper) and pH 7.4 (lower) in the absence (left) and presence (right) of 2 mM DHO. (B) Average current (last 5 ms of voltage pulses) from the myocyte in A, in the absence (open black squares) and presence (open blue triangles) of DHO and at pH 6.0 without (open red circles) and with (open green triangles) DHO; the DHO-sensitive current is plotted as solid black squares (pH 7.4) and solid red circles (pH 6.0). (C) Average DHO-sensitive current density (current normalized by cell capacitance to correct for natural differences in myocyte size) at pH 7.4 (black squares) and at pH 6.0 (red circles). Error bars represent the mean ± SE.