Skip to main content
. Author manuscript; available in PMC: 2015 Apr 10.
Published in final edited form as: Mol Cell. 2014 Mar 27;54(1):166–179. doi: 10.1016/j.molcel.2014.02.025

Figure 7. E90K promotes association of the ERAD factor JB12 with the beclin-1/Vps34 autophagy initiation complex.

Figure 7

(A) Phosphorylation of beclin-1-HA on S93/96 in response to treatment with 10mM LiCl and 15μM Eey1. (B) Phosphorylation of beclin-1-HA on S93/96 in response to E90K expression. (C) Native IP of JB12-FLAG in the presence and absence of beclin-1-HA and E90K. (D) Native IP of JB12-FLAG in the presence and absence of beclin-1-HA and E90K to look at interactions of JB12 with phosphorylated beclin-1. (E) Native IP of JB12-FLAG in the presence and absence of beclin-1-HA, ATZ, or E90K. (F) Native IP of JB12-FLAG in the presence of E90K and Vps34-myc. Western blots of samples from Cos-7 cells are depicted in all panels, see the supplemental experimental procedures for details. See also Figure S7.