Fig. 5.

Effects of anti-TLQP21 on nerve injury-induced hypersensitivity. A, Intrathecal pretreatment with anti-TLQP21 attenuated the development of tactile hypersensitivity after spared nerve injury (SNI). Mice were injected with saline, anti-TLQP21 (anti-TL21, 150 ng/5 μL), or normal rabbit IgG (NR-IgG, 150 ng/5 μL) immediately prior to SNI or sham surgery. On day 2 after surgery, the mechanical withdrawal thresholds of anti-TLQP21 pretreated but not NR-IgG pretreated, SNI mice were significantly different from those of saline pretreated SNI mice. Anti-TLQP21 did not alter the thresholds of sham operated mice compared to saline pretreated sham mice (Saline pretreated SNI vs anti-TLQP21 pretreated SNI, p < 0.0001; Saline pretreated sham vs anti-TLQP21 pretreated sham, p > 0.05; Two-Way ANOVA for repeated measures, Bonferroni post-hoc test; mean +/− SE, n = 7–9 per group). B, Time course of changes in hypersensitivity after pretreatment with anti-TLQP21. Ipsilateral to SNI, the mechanical withdrawal thresholds of anti-TLQP21-pretreated SNI mice, but not mice pretreated with normal rabbit IgG, were significantly different from those of saline-pretreated SNI mice for up to 14 days after nerve injury (Day 2, p < 0.01; Days 6 and 14, p < 0.05; Two-Way ANOVA for repeated measures, Bonferroni post-hoc test; mean +/− SE, n = 4 per group). C, Treatment with anti-TLQP21 attenuated established hypersensitivity. Twelve days after SNI, mice were injected intrathecally with saline, anti-TLQP21 (150 ng/5 μL), or normal rabbit IgG (150 ng/5 μL). The mechanical withdrawal thresholds of anti-TLQP21-treated, but not normal rabbit IgG-treated SNI mice were significantly different from those of saline-treated SNI mice 3 and 24 h after injection (p < 0.0001 and p < 0.05, respectively; Two-Way ANOVA for repeated measures, Bonferroni post-hoc test; mean +/− SE, n = 4 per group).