Figure 3.

Results obtained with AutoTT are not limited by image quality as compared to global thresholding or FFT of unprocessed images. (A) Comparison of AutoTT analysis of original (image a) and brightness-enhanced images (image b). Image b is derived from image a with the brightness artificially enhanced. (B) Images a and b in (A) were converted from grayscale images to binary images and the T-tubule density determined using global thresholding. A rectangular region of each image is shown. (C) FFT was directly applied to the original image a and brightness-enhanced image b. The inset shows the sampling line (yellow dashed line) used for profiling the FFT magnitude. To see this figure in color, go online.