Table 3.
Virus isolation and PCR results for the H5N1 avian influenza virus infected wild birds detected in Hong Kong in 2006–2007
| Viruses isolated | Species of bird | H5N1 Clade / cluster | Samples tested H5 Positive in VI and PCR * | VI # | H5 PCR | M PCR | N1 PCR | AF H5 PCR | AF M PCR | AF N1 PCR |
|---|---|---|---|---|---|---|---|---|---|---|
| 1. D-06-0075 | Magpie Robin | 2.3.4 /6 | C&T, B&L, I | + | + | + | + | + | + | + |
| 2. D-06-0366 | Magpie Robin | 2.3.4 /6 | T,L,B,Li,S,K,H,M | + | + | + | + | + | + | + |
| 3. D-06-0540 | Crested Myna | 2.3.4 /5 | C&T, L, B | + | + | + | + | + | + | + |
| 4. D-06-0645 | Common Magpie | 2.3.4 /1 | C&T, L, B | + | + | + | + | + | + | + |
| 5. D-06-0718 | Little Egret | 2.3.4 /6 | C&T, L, B | + | B + | B + | B + | |||
| 6. D-06-1038 | Japanese White Eye | 2.3.4 /4 | T, B, I | + | + | + | + | + | + | + |
| 7. D-06-2125 | Common Magpie | 2.3.4 /4 | T,L,B,Li,S,K,H,P | + | C,T+ | C,T+ | C,T+ | |||
| 8. D-06-2256 | Common Magpie | 2.3.4 /4 | T,L,B,I | + | + | + | + | + | + | + |
| 9. D-06-2454 | Munia | 2.3.4 /4 | L,B | + | + | + | + | + | + | + |
| 10. D-06-2469 | White-backed Munia | 2.3.4 /4 | T,L,B | + | + | + | + | + | + | + |
| 11. D-06-2512 | Large-billed Crow | 2.3.4 /4 | C&T, L, B | + | + | + | + | + | + | + |
| 12. D-06-2648 | House Crow | 2.3.4 /4 | T,L,B,Li,S,K,H,P | + | B,L,T,I + | B,L,T,I + | B,L,T,I + | |||
| 13. D-06-2858 | House Crow | 2.3.4 /4 | C,T,L,B,S | + | + | + | + | |||
| 14. D-06-3033 | Common Magpie | 2.3.4 /4 | C&T,L,B,S,I | + | + | + | + | + | + | + |
| 15. D-06-5211 | Peregrine Falcon | 2.3.2 | C&T,L,B,S,K,H | + | + | + | + | + | + | + |
| 16. D-07-0045 | Scaly-breasted Munia | 2.3.4 /2 | C&T,T | + | + | + | + | |||
| 17. D-07-0458 | Crested Goshawk | 2.3.4 /2 | C&T,L,B,S,I | + | + | + | + | |||
| 18. D-07-0719 | House Crow | 2.3.4 /7 | C&T,L,S | + | C&T S + |
C&T + |
C&T S + |
+ | + | + |
| 19. D-07-0737 | Japanese White Eye | 2.3.4 /7 | C&T,L | + | T,L + | T,L + | ||||
| 20. D-07-0828 | White-backed Munia | 2.3.4 /7 | C&T,L,B,Li,I,S, | + | + | + | + | + | + | + |
| 21. D-07-1143 | Peregrine Falcon | 2.3.2 | C&T,L,B,H,P | + | + | + | + | |||
| 22. D-07-1203 | House Crow | 2.3.4 /7 | C&T | + | + | + | + | + | + | + |
| 23. D-07-1993 | Blue Magpie | 2.3.4 /7 | C&T,L,B,H,K,Li | + | − | + | C&T B,H+ |
− | + | + |
| 24. D-07-2065 | Silver-eared mesia #1 | 2.3.4 /7 | C&T,L&H&T,B, K&S,I&Li | + | B,L,T,H+ | + | + | +/−B | + | + |
| 24. D-07-2065 | Silver-eared mesia #2 | 2.3.4 /7 | C&T, L&H&T,B, S,I | + | B,I,L K,S+ |
+ | + | +/−B | + | + |
| 25. D-07-2372 | Common Kestrel | 2.3.4 /7 | C&T,L,B,H,K,P,S | + | − | + | + | − | + | + |
| 26. D-07-2433 | Scaly-breasted Munia | 2.3.4 /3 | C&T, L, B | + | + | + | + | + | + | + |
| 27. D-07-2442 | Chestnut Munia | 2.3.4 /3 | C&T, T, I | + | + | + | + | + | + | + |
| 28. D-07-2572 | Scaly-breasted Munia | 2.3.4 /7 | C&T,L,B,H, I&L&K&S | + | C&T B,I + |
+ | + | +/−B, L,H,I | + | + |
| 29. D-07-2762 | Long-tailed shrike | 2.3.4 /2 | C&T,L,B,H,K | + | + | + | + | + | + | + |
| 30. D-07-4925 | Red-billed Starling | 2.3.2 | C&T,L,K,S | + | + | + | + | + | + | + |
| 31. D-07-5052 | Common Magpie | 2.3.2 | C&T,L,B | + | + | + | + | + | + | + |
| 32. D-07-5288 | House Crow | 2.3.2 | C&T,L,B,K | + | + | L,B, K + | + | + | + | + |
*
C cloacal swab, T tracheal swab, L lung, B brain, H heart, S spleen, K kidney, Li liver, P pancreas, I intestines; link by & indicates pooled samples tested
#
VI virus isolation in chicken embryos; H5 PCR is RRT-PCR for H5 gene; M PCR is RRT-PCR for Matrix gene; N1 PCR is conventional RT-PCR for N1 neuraminidase gene; AF allantoic fluid harvest; Scored as (+) positive if all tissues tested were positive; (+/−) for the AF H5 PCR results indicates the tissues that were only positive using the CSIRO H5 primer/probe set; (−) negative; blank space indicates not tested by this test.