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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Struct Biol. 2014 Mar 5;186(1):8–18. doi: 10.1016/j.jsb.2014.02.016

Figure 1.

Figure 1

Data-driven, pipeline approach for real-time, high-resolution 3D reconstruction. The workflow highlights the two pipelines that the approach includes, and the major computational steps in each. Pipeline 1 processes micrographs separately, immediately after each is recorded, to compile a stack of particle images and determine their origin/orientation parameters. Pipeline 2, running in parallel with Pipeline 1, continuously updates the reconstruction and refines the origin and orientation parameters for all particles currently available, in an iterative manner.