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. Author manuscript; available in PMC: 2014 Oct 7.
Published in final edited form as: Nat Commun. 2014 Apr 7;5:3571. doi: 10.1038/ncomms4571

Figure 3. Mapping the alternate MRL24 binding site in PPARγ.

Figure 3

(a) Comparison of 2D [1H,15N]-TROSY-HSQC spectra for 15N-PPARγ LBD bound to 1 or 2 molecules of MRL24 (black and orange, respectively). (b) Comparison of 2D [1H,13C]-methyl CHD2-detected HSQC data for 2H,13C,15N-PPARγ LBD bound to 1 or 2 molecules of MRL24. (c) NMR chemical shift footprinting reveals a decrease in peak intensity between 3D TROSY-HNCO experiments collected for 2H,13C,15N-PPARγ LBD bound to 1 or 2 molecules of MRL24 (black/pink and orange/grey, respectively, for positive/negative peak amplitudes) and reveals residues affected by the alternate site binding event.