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. Author manuscript; available in PMC: 2014 Oct 7.
Published in final edited form as: Nat Commun. 2014 Apr 7;5:3571. doi: 10.1038/ncomms4571

Figure 7. Alternate site binding of MRL20 to PPARγ bound to an endogenous ligand.

Figure 7

(a) 19F NMR analysis reveals that MRL20 binds to PPARγ LBD covalently bound to endogenous ligands. (b,c) TR-FRET assay demonstrates that alternate site MRL20 binding affects the interaction between (b) TRAP220-2 and (c) NCoR-D2 for PPARγ LBD covalently bound to 15-oxoETE, but not 5-oxoETE. PPARγ LBD protein not covalently bound to an endogenous ligand but exposed to vehicle (ethanol) was used as a control, performed in duplicate and plotted as the average (± s.d.) and fit to a sigmoidal dose response curve.