Protein expression levels of ZO-1, claudin-5, and occludin on ischemic cerebral microvessels after cerebral ischemia reperfusion. Western blots of (a) ZO-1, (c) claudin-5, and (e) occludin. From left to right: sham; M1–M7: MCAO groups after 1, 3, 5, and 7 d of reperfusion; and E1–E7: EA groups after 1, 3, 5, and 7 d of reperfusion. β-Actin served as a loading control. IDV of ZO-1 (b), claudin-5 (d), and occluding (f) are shown. Data (n = 3) is represented as mean ± SD. Δ
P < 0.05 versus the sham group, ★
P < 0.05 versus the MCAO group at the same time points, and *P < 0.05 versus the EA groups at the same time points.