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. Author manuscript; available in PMC: 2014 Jun 25.
Published in final edited form as: Curr Cancer Drug Targets. 2011 Jun;11(5):654–669. doi: 10.2174/156800911795655967

Figure 7. Anchorage-independent growth of H16N2 clones overexpressing AhR.

Figure 7

Single-cell suspensions of different clones were prepared in 0.3% agar media and overlaid onto 0.6% agar layer coated on each well of the six-well plates. Cells were plated in triplicate at two cell densities. Individual cells were visualized microscopically and colony formation was monitored for up to 2 weeks. The numbers of colonies were counted at low magnification (20x) and the scoring was done by three independent observers. Values are means and standard deviations of two independent experiments. A (*) is significantly different from EV control (p<0.05).