Table 5.

Experimental design and results of the CCD.

Run	Type	Moisture	Beef extract	NaH2PO4	Response (Y) (units/mL)
1	Factorial	−1 (60)	−1 (0.1)	1 (0.05)	2175
2	Center	0 (80)	0 (0.2)	0 (0.0375)	2553
3	Center	0 (80)	0 (0.2)	0 (0.0375)	3150
4	Center	0 (80)	0 (0.2)	0 (0.0375)	2868
5	Factorial	−1 (60)	1 (0.3)	1 (0.05)	2592
6	Factorial	1 (100)	−1 (0.1)	1 (0.05)	2916
7	Axial	1.682 (113.64)	0 (0.2)	0 (0.0375)	1522
8	Axial	0 (80)	0 (0.2)	−1.662 (0.0182)	2731
9	Factorial	1 (100)	1 (0.3)	1 (0.05)	3699
10	Axial	0 (80)	−1.682 (0.34)	0 (0.0375)	2463
11	Center	0 (80)	0 (0.2)	0 (0.0375)	3147
12	Factorial	−1 (60)	−1 (0.1)	−1 (0.025)	1372
13	Factorial	−1 (60)	1 (0.3)	−1 (0.025)	2689
14	Axial	0 (80)	−1.682 (0.06)	0 (0.0375)	3012
15	Factorial	1 (100)	1 (0.3)	−1 (0.025)	2182
16	Factorial	1 (100)	−1 (0.1)	−1 (0.025)	2209
17	Center	0 (80)	0 (0.2)	0 (0.0375)	3048
18	Axial	0 (80)	0 (0.2)	1.682 (0.0568)	3334
19	Center	0 (80)	0 (0.2)	0 (0.0375)	2779
20	Axial	−1.682 (46.36)	0 (0.2)	0 (0.0375)	86