Table 1.
Summary of reads and assembly from 454 GS FLX sequencing for Anopheles sinensis
| Library | Raw reads | Average length (bp) | Reads used in assembly* | % reads mapped to contig set | No. contigs | N50 contigs (bp) | Average contig length (bp) | % GC contigs |
|---|---|---|---|---|---|---|---|---|
| Immature stage, colony | 164,503 | 341.1 | 122,661 | 75.45 | 17,398 | 491 | 447 | 45.3 |
| Female adults, colony | 153,568 | 299.0 | 106,268 | 71.1 | 7,415 | 515 | 453 | 44.5 |
| Deltamethrin resistant, field | 171,679 | 363.3 | 124,723 | 73.0 | 13,897 | 524 | 481 | 45.5 |
| Deltamethrin susceptible, field | 134,809 | 304.6 | 91,193 | 68.3 | 10,597 | 464 | 427 | 45.2 |
| Total | 624,559 | 290.8 | 394,254 | 71.9 | 33,411 | 547 | 493 | 45.1 |
*Reads used in assembly is less than the total number of raw reads due to removal of duplicated, ambiguous and short (<50 bp) sequences.