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. 2014 Jun 6;15(1):439. doi: 10.1186/1471-2164-15-439

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© Alexeyenko et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quality parameters of FPs. A, cumulative assembly length of contigs from 360 FPs in the production set longer than a given threshold. Each colored line represents a distribution of total assembled length per pool by taking into account contigs longer than the specified length (0…20 kbp). B, length distribution of contigs that contained both fragments that matched start and end of the vector sequence (from 79 FPs produced with four different vectors). The expected range length was 27…47 Kbp, hence the outliers emerged due to either damaged Fosmids or mis-assembly. C, distribution of coverage across different contigs in pools (from 360 FPs in the production set). Bins of the histogram represent the number of FPs that have coverage not worse than the threshold specified in the color legend. For example, the highest point of the green line tells that there were 170 FPs in which 97% of contigs were covered at least 10x (as average along the contig length). A, B, C.