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. 2013 Sep 27;3:2786. doi: 10.1038/srep02786

Figure 2. Lentivirus infection and Feridex-labeling of MSCs.

Figure 2

(A) Schematic diagram of vector construction. (B) Expression of GDNF mRNA in MSCs after lentivirus infection. (C) Relative abundance of GDNF mRNA analyzed by real-time PCR in GDNF-MSCs at various MOIs. (D) GDNF secretion analyzed by ELISA in GDNF-MSCs at various MOIs. (E) Compared to the two non-infected MSC lines, GDNF secretion was significantly higher in MSCs infected with lenti-GDNF at MOI 10. (F) Stable expression of GDNF for 40 days. (G) Feridex-labeled MSCs appeared yellowish under bring field microscopy and could be visualized by Prussian blue staining. (C–E) *P < 0.05, **P < 0.01).