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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Chem Sci. 2014 Apr 7;5(6):2503–2516. doi: 10.1039/C4SC00316K

Fig 1. Zip7 loss in CbCln6nclf cells is not associated with altered global biometal levels.

Fig 1

A. Representative immunoblot of Zip7 and total ERK expression in cellular homogenates (5μg) harvested from non-treated control and CbCln6nclf mutant cells. B. Densitometry analysis of immunoblots (N=3 biological replicates), normalized to expression of total ERK protein was performed using ImageJ. Values are mean + SEM. ** p<0.01 by Student’s t-test. C–F Total cellular zinc (C), copper (D), iron (E) and calcium (F) concentrations were analysed using ICP-MS. Metal concentrations are normalized to total cellular protein content, as measured by BCA assay, and are expressed as mean + SEM.