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. Author manuscript; available in PMC: 2014 Jun 25.
Published in final edited form as: Dev Dyn. 2011 Sep;240(9):2084–2100. doi: 10.1002/dvdy.22715

Figure 5. Neural crest cells from the level of somites 5-7 [stage-10 embryos] always migrate in the ventral pathway.

Figure 5

Quail neural tubes (labeled with QCPN antibody; green) from somite-level 5-7 were transplanted heterotopically and heterochronically to test for cell autonomous behavior. Neural crest cells are labeled with HNK-1 (red) and the myotome labeled with MF20 (white in D only) to indicate unambiguously the boundary between the dorsolateral and ventral pathways. The boxed areas are shown at higher magnification in the insets. Representative sections are shown from somite-levels 1,2,3 (A-C) or the trunk level (D).

The transplanted neural crest cells from the level of somites 5-7 from stage-10 embryos maintain their migration into the ventral pathway when they are transplanted to the level of somites 1-3 in stage-10 embryo hosts,12 hours later [stage 13] (A-C) or to the trunk level in stage-21 embryo hosts (arrowheads D).

Somite 1 (S1), Somite 2 (S2), Somite 3 (S3), Dermomyotome (DM), Dorsal aorta (DA), Neural tube (NT), Pharynx (Ph).

Scale bar = 100μm.