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. Author manuscript; available in PMC: 2014 Jun 25.
Published in final edited form as: Dev Dyn. 2011 Sep;240(9):2084–2100. doi: 10.1002/dvdy.22715

Figure 6. Neural crest cells from the level of somites 1-2 [stage-10 embryos] transplanted to the level of somites 5-7 [stage-10 embryos] migrate into the stomach and not into the heart.

Figure 6

To test whether neural crest cells from somite-level 1-2, which normally reach the lung buds, can migrate more posteriorly to the stomach, a quail neural tube (labeled with QCPN, green) from somite level 1-2 was grafted to the axial level of somites 5-7 in a stage-10 embryo. Neural crest cells were labeled with HNK-1 (red) and the myotome labeled with MF20 (cyan). Forty-eight hours later [stage 20], the transplanted neural crest cells (QCPN-positive) migrated posteriorly into the stomach (arrowheads B, BB), which is normally populated by neural crest cells from the level of somites 5-7. However, the transplanted neural crest cells did not migrate anteriorly into the pharyngeal arches 4-6 and the outflow tract, which they normally populate (A, AA). Dorsal aorta (DA), Cardinal vein (CV). Scale bar = 100μm.