Figure 2. Comparison of homology length of donor DNA to induce HR in ZFN mediated gene targeting.

(a) Construction of donor vectors with 5 different homology lengths. (b) Quantitative PCR analysis for detection of ZFN/donor DNA mediated targeted cells. For donor DNA construct and PCR primer sets, see the Method. For PCR analysis of ZFN-assisted homologous recombination events, we used the same methods for screening and analysis of knockout colony using Neo A and CMAH B primers located outside of the recombination region (Supplementary table 1).