Figure 5. Sialyltransferase gene expression levels in control, monoallelic, and biallelic CMAH KO pigs.

(a) Electrophoretic analysis of RT-PCR from control, monoallelic, and biallelic CMAH KO pig-derived fibroblast cells. (b) Comparison of sialyltransferase gene expression in control-, monoallelic-, and biallelic-derived pig fibroblast cells by real-time RT-PCR. (c) Quantification of real-time RT-PCR analysis in control-, monoallelic-, and biallelic-pig fibroblast cells. All RT-PCR reactions were conducted in triplicate and normalized with pig actin mRNA. Each of monoallelic- and biallelic-pig relative values is presented as an n-fold expression difference compared to the control pig, which was set as 1. *P < 0.05 and **P < 0.001.