Table 1. Efficiency of CMAH targeting in fibroblast cells using ZFN and donor DNA. Female cells showed decrease efficiency of the targeting event.

Source of cells	Source of ZFN	No. of cells transfected	No. of neo-positive colonies analyzed by PCR	No. of positive colonies (%)
Male	DNA	5 × 106	64	19 (29.7)
	mRNA	5 × 106	48	15 (31.3)
	Sub-total		112	34 (30.4)
Female	DNA	5 × 106	39	3 (7.7)
	mRNA	5 × 106	62	2 (3.2)
	Sub-total		101	5 (5.0)
Total			213	39 (18.3)