Figure 1.

A proteomic study of the effects of EGCG on H₂O₂-induced oxidative stress in H9c2 cells. (a) MTT assay of cell viability after incubation with 0, 50, 100, 200, 400 and 1000 μM H₂O₂ for 30 min (b) MTT assay of cell viability after incubation with 0, 5, 10, 20, 50 and 100 μM EGCG for 30 min (c) H9c2 cells were pre-treated with 10 and 20 μM EGCG for 30 min followed by 400 μM H₂O₂ for 30 min, cell viability was measured by MTT assay (d) Phase contrast microscopy showing cell morphology of H9c2 cells in the condition of control (left), 400 μM H₂O₂ exposure for 30 min (middle), and 20 μM EGCG pre-treatment for 30 min followed by 400 μM H₂O₂ exposure for 30 min (right). (Scale Bar = 200 μm). (e) 2-DE gel images showing protein distribution in the condition of control (left), 400 μM H₂O₂ exposure for 30 min (middle), and 20 μM EGCG pre-treatment for 30 min followed by 400 μM H₂O₂ exposure for 30 min (right). In a, the values are the mean ± SEM, with ***p < 0.001.