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. 2014 Jun 9;21(1):56. doi: 10.1186/1423-0127-21-56

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Copyright © 2014 Chen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of H₂O₂and EGCG on PI3K/AKT/GSK3β signaling pathway. (a) Representative result of Inpp4b protein level in 2-DE gel and mRNA expression (b) Western blotting (right) and quantitative analysis (left) showing effects of EGCG on Inpp4b, p-AKT (S473), AKT, p-GSK-3β (S9), cyclin D1 in H₂O₂-induced H9c2 cells. (c) MTT assay showing the improvement of H₂O₂-suppressed cell viability by EGCG and/or GSK-3β inhibitor, SB216763 pre-treatment in H₂O₂-induced H9c2 cells. (d) Western blotting (right) and quantitative analysis (left) showing effects of EGCG and/or GSK-3β inhibition by GSK-3β inhibitor, SB216763, on the phosphorylation of Akt (S473), GSK-3β (S9) and cyclin D1 in H₂O₂-induced H9c2 cells. All values are the mean ± SEM, with *p < 0.05; **p < 0.01; ***p < 0.001.