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. 2014 Jun 12;14:426. doi: 10.1186/1471-2407-14-426

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Copyright © 2014 Wong et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of celecoxib and aspirin on plasma estradiol concentration, uterine weight and ERα expression. Blood was drawn from the animals at sacrifice. Serum estradiol concentration was quantified by ELISA (A). Sample labeled as AD is androstenedione. Uterine of the experimental animals were dissected and weighed at sacrifice as shown in B. Western blot analysis of ERα in xenograft samples was analyzed by immunoblotting (C) and the optical density measurements were shown in the lower panel. Groups labeled as AD, AD+celecoxib, and AD+aspirin are the mice treated with androstenedione, androstenedione and celecoxib, and androstenedione and aspirin, respectively. Values are means ± SEM, n=6 to 8.The data was analyzed by One-way ANOVA, followed by Tukey’s Multiple Comparison test when P<0.05. Means labeled with different letter are significantly different (order: b>a).