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. 2014 May 27;13:125. doi: 10.1186/1476-4598-13-125

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Copyright © 2014 Bachmann et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DTX3L-mediated cell migration of PC3 cells might also be dependent on STAT3. (A-F) PC3-siSTAT3 single knockdown cells (A), PC3-siDTX3L/siSTAT3 (B), PC3-siSTAT1/siSTAT3 (C) double knockdown cells and PC3-siDTX3L/siSTAT1/siSTAT3 (D) triple knockdown cells were seeded into 6-well plates and treated as described in Material and Methods. At 0, 12, 24, and 36 h photographs were made and quantified as described in Material and Methods. Values represent the mean of three independent experiments and the error bars represent the SE. Statistical analysis was performed using the Student's t test. *P < 0.05, **P < 0.001 and ***P < 0.0001.