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Phylogeny of methanogens. The neighbor-joining tree was constructed on the basis of 16S rRNA gene sequences of previously known methanogens (19) and Methanomassiliicoccales-related strains (3, 9, 22) using the ARB software (15). The 16S rRNA gene sequences of the Thermodesulfobacterium (AF418169, AF334601, NR_025146, NR_029311) were used as outgroups (not shown). The bar indicates 10% base substitution.
